Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection
Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
| Fibroblast (3T3, COS-7) | Myoblast (C2C12, L6 CRL-1458) |
| Hepatocyte (HepG2, HuH7) | Erythroleukemic cell (K562) |
| Breast carcinoma (MCF7, Hs578T) | Prostate carcinoma (LNCap) |
| Lung carcinoma (A549, NCI-H460) | Osteosarcoma (U-2 OS, Saos-2) |
| Colon carcinoma (Caco2, SW480) | Pancreatic carcinoma (PANC-1) |
| Skin melanoma (SK-MEL-28) |
View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >
Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).