(APPDE300) α+ Solution™ Plasmid Extraction Mini Kit
※ Principle : mini spin column (silica matrix)
※ Sample size : 1 ~ 3 ml
※ Size of plasmid or construct : < 15 kb
※ Operation time : < 25 minutes
※ Typical Yield : 20 ~ 30 μg
※ Binding capacity : 60 μg/ column
※ Column applicability : centrifugation and vaccum
Important Notes:
※ Store RNase A at -20 °C upon recipit of kit.
※ Add 0.5 ml of PD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the PD1 bottle, mix well by vortexing and store the PD1 buffer at 4 °C.
※ If precipitates have formed in PD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.
※ Preparation of Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.
※ Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g
#PLASMID DNA EXTRACTION KIT #DNA KIT
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